BM-MSC

Analysis of cells from bone marrow (BM) processed by CellEffic BM

Recoveries of mesenchymal stem/stromal cells (MSCs) and erythrocytes removal by CellEffic BM

 
30mL BM processed
(n=5 Av/SD)
3mL of erythrocytes depleted BM processed
(n=5 Av/SD)
1mL BM processed
(n=5 Av/SD)
Fold increase CFU-F compared to Ficoll
2.55/1.24
 
2.51/1.15
MSC Recovery Ratio (%)
 
92.7/12.9
 
Erythrocyte Removal Ratio (%)
 
 
93.7/0.8

CFU-F: colony-forming units-fibroblast

Analysis of markers for MSCs and the blood system on cells isolated from bone marrow by CellEffic BM (compared to cells processed by Ficoll)

Surface Marker Analysis

Marker analysis BM

Flow cytometric analysis performed at P3. N=5; Mean/SD are shown.

Differentiation ability of cells from bone marrow processed by CellEffic BM

MSCs (stimulated at P2) isolated by CellEffic BM are able to differentiate towards osteogenic, chondrogenic and adipogenic lineages as are the control cells processed by Ficoll (data on file).